沉降菌培养温度

莫大爷

沉降菌培养温度到底需不需要22.5℃培养两天，再32.5℃培养3天？因为我看到16294里面写的是30-35度培养不少于2天，但是有了解到好多药企都是高低温培养。想问问各位老师的看法

莫大爷

火舞精灵 发表于 2021-04-16 14:33
细菌培养温度是30~35，真菌培养温度是20~25，TSA培养基即可以培养细菌也可以培养真菌，做一次两个温度培养，两个结果都有了，以前中国药典内细菌用营养琼脂，真菌用玫瑰红娜琼脂，做个沉降菌都得用两种培养基，后来和国际接轨了，就成了TSA，一种培养基可以出具两个结果。为什么要出两个结果？检察官要的是环境监测的结果，不是单独细菌或真菌的，是两个一起的。

有道理！

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野风吹北

30-35培养3天啊。TSA为啥要用低温培养呢？

yuansoul

野风吹北 发表于 2021-4-16 12:51
30-35培养3天啊。TSA为啥要用低温培养呢？

tsb  正在思考

莫大爷

野风吹北 发表于 2021-4-16 12:51
30-35培养3天啊。TSA为啥要用低温培养呢？

为了让真菌生长，关键是很多药企都是高低温培养，我现在有点犹豫

瓶瓶

莫大爷 发表于 2021-4-16 13:06
为了让真菌生长，关键是很多药企都是高低温培养，我现在有点犹豫

以法规为依据

东皇太心

听说USP是这样做的，你可以去打听下。
说到这我也有个问题：
如果产品是中美双报的，那我选择USP的方法还是中国的方法？还是两种方法都要？。求解答

火舞精灵

细菌培养温度是30~35，真菌培养温度是20~25，TSA培养基即可以培养细菌也可以培养真菌，做一次两个温度培养，两个结果都有了，以前中国药典内细菌用营养琼脂，真菌用玫瑰红娜琼脂，做个沉降菌都得用两种培养基，后来和国际接轨了，就成了TSA，一种培养基可以出具两个结果。为什么要出两个结果？检察官要的是环境监测的结果，不是单独细菌或真菌的，是两个一起的。

光风霁月

你可以看看USP<1116>,论坛里面可以找到USP35-1116，在Selection of Culture Conditions
Time and incubation temperatures are set once the appropriate media have been selected. Typically, for general microbiological growth media such as SCDM, incubation temperatures in the ranges of approximately 20℃–35℃ have been used with an incubation time of not less than 72 hours. Longer incubation time may be considered when contaminants are known to be slow growing. The temperature ranges given above are by no means absolute. Mesophilic bacteria and moldcommon to the typical facility environment are generally capable of growing over a wide range of temperatures. For many mesophilic organisms, recovery is possible over a range of approximately 20℃. In the absence of confirmatory evidence, microbiologists may incubate a single plate at both a low and a higher temperature. Incubating at the lower temperature first may compromise the recovery of Gram-positive cocci that are important because they are often associated with humans.

莫大爷

光风霁月 发表于 2021-04-16 16:31
本帖最后由 光风霁月 于 2021-4-16 17:15 编辑

你可以看看USP&lt;1116&gt;,论坛里面可以找到USP35-1116，在Selection of Culture Conditions
Time and incubation temperatures are set once the appropriate media have been selected. Typically, for general microbiological growth media such as SCDM, incubation temperatures in the ranges of approximately 20℃–35℃ have been used with an incubation time of not less than 72 hours. Longer incubation time may be considered when contaminants are known to be slow growing. The temperature ranges given above are by no means absolute. Mesophilic bacteria and moldcommon to the typical facility environment are generally capable of growing over a wide range of temperatures. For many mesophilic organisms, recovery is possible over a range of approximately 20℃. In the absence of confirmatory evidence, microbiologists may incubate a single plate at both a low and a higher temperature. Incubating at the lower temperature first may compromise the recovery of Gram-positive cocci that are important because they are often associated with humans.

好的，谢谢

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loveecoli

单温培养，高低温培养（还涉及到先高温还是先低温）都有使用的，也都有相关的法规指南出处，主要还是要有科学的数据说明你的培养条件是合适的

光风霁月

莫大爷 发表于 2021-4-16 20:34
好的，谢谢

这里面给出的培养条件选择的这一段，最后两句：
在没有确凿证据的情况下，微生物学家可能需要在较低温度和较高温度对碟子进行培养。先在较低温度培养可能不利于革兰氏阳性球菌的回收，由于这类菌通常与人类有关所以这一点很重要。
compromise这里应该是不利于，降低之类的意思。看了一些中文翻译稿，这里翻译成 首先在低温培养，确保比较重要的与人类密切相关的革兰氏阳性球菌的生长。

酒酿汤圆

火舞精灵 发表于 2021-4-16 14:33
细菌培养温度是30~35，真菌培养温度是20~25，TSA培养基即可以培养细菌也可以培养真菌，做一次两个温度培养 ...

那我想问一下，是先高温培养48h。后低温培养72h？那这种情况，会不会导致霉菌不好生长？高温会导致水分缺失

轻舟之轻

酒酿汤圆 发表于 2021-6-1 16:25
那我想问一下，是先高温培养48h。后低温培养72h？那这种情况，会不会导致霉菌不好生长？高温会导致水分缺 ...

蹲一个回答  我们公司现在就是这样培养的   感觉先高温还是低温说法很多

小金库

感谢蒲友分享。

nongzi

学习了学习了，学无止境啊